Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12857/115808
Title: NELL1, whose high expression correlates with negative outcomes, has different methylation patterns in alveolar and embryonal rhabdomyosarcoma
Authors: Tombolan, Lucia 
Poli, Elena 
Martini, Paolo 
Zin, Angelica 
Romualdi, Chiara 
Bisogno, Gianni 
Lanfranchi, Gerolamo 
Keywords: DNA methylation;NELL1;RRBS;rhabdomyosarcoma;sequencing
Keywords Plus: DNA METHYLTRANSFERASE EXPRESSION;GENE;FUSION;PAX3-FKHR;PROGNOSIS;REVEALS;ISLANDS;DEFINES;COMMON;PAX3
Mesh headings: DNA Methylation;Nerve Tissue Proteins;Rhabdomyosarcoma, Alveolar;Rhabdomyosarcoma, Embryonal;Up-Regulation
Secondary Mesh headings: Cell Line, Tumor;Child;Child, Preschool;Epigenesis, Genetic;Female;Gene Expression Regulation, Neoplastic;Humans;Male;Oncogene Proteins, Fusion;Prognosis;Translocation, Genetic
Issue Date: 16-May-2017
Publisher: IMPACT JOURNALS LLC
Journal: Oncotarget 
Abstract: 
Rhabdomyosarcoma (RMS), which represents the most frequent soft tissue sarcoma in pediatric populations, is classified into two major subtypes: embryonal RMS (ERMS) and alveolar RMS (ARMS). ARMS subtype, which shows greater aggressiveness and proneness to metastasis with respect to ERMS, are characterized, in about 75% of cases, by specific chromosomal translocations that involve PAX and FOXO1 genes. Many findings have demonstrated that PAX/FOXO1-positive ARMS have a worse prognosis than PAX/FOXO1-negative ones and that distinct molecular features characterize RMS with different gene fusion statuses. DNA methylation, which presently represents a challenging research area, is involved in the modulation of gene expression.We performed a genome-wide DNA methylation analysis using reduced-representation bisulfite sequencing (RRBS) in RMS samples and we found that fusion-positive alveolar and embryonal subgroups have different DNA methylation signatures and that ARMS fusion-positive subtypes are characterized by overall hypomethylation levels. While NELL1 was found to be hypomethylated and transcriptionally enhanced in RMS alveolar subtypes, high NELL1 expression levels, which proved to be correlated with negative RMS prognostic factors such as fusion status and histology (P < 0.0001), were found to discriminate between RMS patients with different outcomes (P < 0.05).In conclusion, our results demonstrated that different DNA methylation patterns distinguish between different RMS subgroups and they suggest that epigenetic signatures could be useful for risk stratification of patients.
URI: http://hdl.handle.net/20.500.12857/115808
DOI: 10.18632/oncotarget.16526
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