Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12857/115853
Title: Hyper-activation of Notch3 amplifies the proliferative potential of rhabdomyosarcoma cells
Authors: De Salvo, Maria
Raimondi, Lavinia
Vella, Serena
Adesso, Laura
Ciarapica, Roberta
Verginelli, Federica
Pannuti, Antonio
Citti, Arianna
Boldrini, Renata
Milano, Giuseppe M
Cacchione, Antonella
Ferrari, Andrea
Collini, Paola
Rosolen, Angelo 
Bisogno, Gianni 
Alaggio, Rita 
Inserra, Alessandro
Locatelli, Mattia
Stifani, Stefano
Screpanti, Isabella
Miele, Lucio
Locatelli, Franco
Rota, Rossella
Keywords Plus: NF-KAPPA-B;ALVEOLAR RHABDOMYOSARCOMA;INHIBITION;EXPRESSION;DIFFERENTIATION;SURVIVAL;PATHWAY;PROTEIN
Mesh headings: Cell Proliferation;Gene Expression Regulation, Neoplastic;Receptors, Notch;Rhabdomyosarcoma, Alveolar;Rhabdomyosarcoma, Embryonal;Signal Transduction
Secondary Mesh headings: Cell Line, Tumor;Humans;Ki-67 Antigen;Oncogene Proteins, Fusion;Paired Box Transcription Factors;Receptor, Notch1;Receptor, Notch3
Issue Date: 2014
Publisher: PUBLIC LIBRARY SCIENCE
Journal: PloS one 
Abstract: 
Rhabdomyosarcoma (RMS) is a pediatric myogenic-derived soft tissue sarcoma that includes two major histopathological subtypes: embryonal and alveolar. The majority of alveolar RMS expresses PAX3-FOXO1 fusion oncoprotein, associated with the worst prognosis. RMS cells show myogenic markers expression but are unable to terminally differentiate. The Notch signaling pathway is a master player during myogenesis, with Notch1 activation sustaining myoblast expansion and Notch3 activation inhibiting myoblast fusion and differentiation. Accordingly, Notch1 signaling is up-regulated and activated in embryonal RMS samples and supports the proliferation of tumor cells. However, it is unable to control their differentiation properties. We previously reported that Notch3 is activated in RMS cell lines, of both alveolar and embryonal subtype, and acts by inhibiting differentiation. Moreover, Notch3 depletion reduces PAX3-FOXO1 alveolar RMS tumor growth in vivo. However, whether Notch3 activation also sustains the proliferation of RMS cells remained unclear. To address this question, we forced the expression of the activated form of Notch3, Notch3IC, in the RH30 and RH41 PAX3-FOXO1-positive alveolar and in the RD embryonal RMS cell lines and studied the proliferation of these cells. We show that, in all three cell lines tested, Notch3IC over-expression stimulates in vitro cell proliferation and prevents the effects of pharmacological Notch inhibition. Furthermore, Notch3IC further increases RH30 cell growth in vivo. Interestingly, knockdown of Notch canonical ligands JAG1 or DLL1 in RMS cell lines decreases Notch3 activity and reduces cell proliferation. Finally, the expression of Notch3IC and its target gene HES1 correlates with that of the proliferative marker Ki67 in a small cohort of primary PAX-FOXO1 alveolar RMS samples. These results strongly suggest that high levels of Notch3 activation increase the proliferative potential of RMS cells.
URI: http://hdl.handle.net/20.500.12857/115853
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0096238
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