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|Title:||Surface plasmon resonance based on molecularly imprinted nanoparticles for the picomolar detection of the iron regulating hormone Hepcidin-25||Authors:||Cenci, Lucia
Bossi, Alessandra Maria
|Keywords:||Biosensor;Hepcidin;Iron metabolism;Molecularly imprinted polymers;Nanoparticles;Surface plasmon resonance||Keywords Plus:||SCIENCE-AND-TECHNOLOGY;POLYMER NANOPARTICLES;ANTIMICROBIAL ACTIVITY;OPTICAL-FIBER;RECOGNITION;BINDING;SENSOR;QUANTIFICATION;PROTEINS;AFFINITY||Mesh headings:||Hepcidins;Molecular Imprinting;Nanoparticles;Surface Plasmon Resonance||Secondary Mesh headings:||Humans;Iron;Limit of Detection||Issue Date:||27-Aug-2015||Publisher:||BMC||Journal:||Journal of nanobiotechnology||Abstract:||
Molecularly imprinted polymer (MIP) technique is a powerful mean to produce tailor made synthetic recognition sites. Here precipitation polymerization was exploited to produce a library of MIP nanoparticles (NPs) targeting the N terminus of the hormone Hepcidin-25, whose serum levels correlate with iron dis-metabolisms and doping. Biotinylated MIP NPs were immobilized to NeutrAvidin™ SPR sensor chip. The response of the MIP NP sensor to Hepcidin-25 was studied.
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