Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12857/116515
Title: The Importance of Distinguishing Menstrual and Peripheral Blood in Forensic Casework: A Case Report
Authors: Tozzo, Pamela 
Nespeca, Patrizia 
Spigarolo, Gloria 
Caenazzo, Luciana 
Keywords: case report;forensic genetics;genetic profile;mRNA typing;sexual assault
Keywords Plus: MESSENGER-RNA;IDENTIFICATION;SECRETIONS;DNA
Mesh headings: Blood Stains;Menstruation;Sex Offenses
Secondary Mesh headings: Adolescent;Alcoholic Intoxication;Clothing;DNA Fingerprinting;Electrophoresis, Capillary;Female;Humans;Microsatellite Repeats;Multiplex Polymerase Chain Reaction;Specimen Handling;Unconsciousness;Vagina;Vulva
Issue Date: Dec-2018
Publisher: LIPPINCOTT WILLIAMS & WILKINS
Journal: The American journal of forensic medicine and pathology 
Abstract: 
This report presents a case of an 18-year-old woman found unconscious by police officers outside a club suspected to be victim of a sexual assault. She was not able to give information about the dynamics of the assault, since she was under the effect of a high amount of alcohol. She affirmed to be virgin.The forensic genetic analyses were performed on vaginal and rectal swabs as well as bloody-like traces present on the slip. DNA and RNA analysis were performed using a multiplex for 15 autosomal short tandem repeat markers and of 19 primers specific for different tissues, respectively.No male DNA was identified on vaginal and rectal swabs and on blood spots on the underwear. Blood, vaginal, and skin markers were scored observed in the samples obtained from slip traces and vaginal swab.The forensic genetic analysis supports the proposition that the victim had a sexual intercourse, in the absence of male biological material, in a case where the victim, altered by high blood alcohol levels, was unable to provide information.
URI: http://hdl.handle.net/20.500.12857/116515
ISSN: 01957910
DOI: 10.1097/PAF.0000000000000414
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