Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12857/116573
Title: Protein Corona Mediated Uptake and Cytotoxicity of Silver Nanoparticles in Mouse Embryonic Fibroblast
Authors: Barbalinardo, Marianna
Caicci, Federico 
Cavallini, Massimiliano
Gentili, Denis
Keywords: cell uptake;cytotoxicity;protein corona;serum-free culture;silver nanoparticles
Keywords Plus: SELF-ASSEMBLED MONOLAYERS;BIOLOGICAL IDENTITY;GOLD NANOPARTICLES;CELLULAR UPTAKE;TOXICITY;CELLS;SURFACE;NANOMATERIALS;IMPACT;GENOTOXICITY
Mesh headings: Embryo, Mammalian;Fibroblasts;Metal Nanoparticles;Protein Corona;Silver
Secondary Mesh headings: Adsorption;Animals;Blood Proteins;Cell Death;Cell Survival;Mice;NIH 3T3 Cells
Issue Date: 2018
Publisher: WILEY-V C H VERLAG GMBH
Journal: Small (Weinheim an der Bergstrasse, Germany) 
Abstract: 
Medical applications of nanoparticles (NPs) require understanding of their interactions with living systems in order to control their physiological response, such as cellular uptake and cytotoxicity. When NPs are exposed to biological fluids, the adsorption of extracellular proteins on the surface of NPs, creating the so-called protein corona, can critically affect their interactions with cells. Here, the effect of surface coating of silver nanoparticles (AgNPs) on the adsorption of serum proteins (SPs) and its consequence on cellular uptake and cytotoxicity in mouse embryonic fibroblasts are shown. In particular, citrate-capped AgNPs are internalized by cells and show a time- and dose-dependent toxicity, while the passivation of the NP surface with an oligo(ethylene glycol) (OEG)-alkanethiol drastically reduces their uptake and cytotoxicity. The exposure to growth media containing SPs reveals that citrate-capped AgNPs are promptly coated and stabilized by proteins, while the AgNPs resulting from capping with the OEG-alkanethiol are more resistant to adsorption of proteins onto their surface. Using NIH-3T3 cultured in serum-free, the key role of the adsorption of SPs onto surface of NPs is shown as only AgNPs with a preformed protein corona can be internalized by the cells and, consequently, carry out their inherent cytotoxic activity.
URI: http://hdl.handle.net/20.500.12857/116573
ISSN: 16136810
DOI: 10.1002/smll.201801219
Appears in Collections:Articles

Show full item record

PubMed Central
Citations 50

17
Last Week
0
Last month
1
checked on Aug 8, 2022

SCOPUSTM   
Citations 50

21
checked on Aug 31, 2020

WEB OF SCIENCETM
Citations

57
checked on Aug 7, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.