Please use this identifier to cite or link to this item:
|Title:||Construction and Characterization of the Mycobacterium tuberculosis sigE fadD26 Unmarked Double Mutant as a Vaccine Candidate||Authors:||Hernandez-Pando, Rogelio
Shin, Sung Jae
Cioetto Mazzabò, Laura
|Keywords:||BCG;SigE;sigma factor;tuberculosis;vaccine||Mesh headings:||Ligases;Mycobacterium tuberculosis;Sigma Factor;Tuberculosis Vaccines;Tuberculosis, Pulmonary||Secondary Mesh headings:||Animals;Bacterial Proteins;Disease Models, Animal;Guinea Pigs;Mice;Vaccines, Attenuated;Virulence||Issue Date:||2019||Journal:||Infection and immunity||Abstract:||
Despite the great increase in the understanding of the biology and pathogenesis of Mycobacterium tuberculosis achieved by the scientific community in recent decades, tuberculosis (TB) still represents one of the major threats to global human health. The only available vaccine (Mycobacterium bovis BCG) protects children from disseminated forms of TB but does not effectively protect adults from the respiratory form of the disease, making the development of new and more-efficacious vaccines against the pulmonary forms of TB a major goal for the improvement of global health. Among the different strategies being developed to reach this goal is the construction of attenuated strains more efficacious and safer than BCG. We recently showed that a sigE mutant of M. tuberculosis was more attenuated and more efficacious than BCG in a mouse model of infection. In this paper, we describe the construction and characterization of an M. tuberculosissigE fadD26 unmarked double mutant fulfilling the criteria of the Geneva Consensus for entering human clinical trials. The data presented suggest that this mutant is even more attenuated and slightly more efficacious than the previous sigE mutant in different mouse models of infection and is equivalent to BCG in a guinea pig model of infection.
|Appears in Collections:||Articles|
Show full item record
checked on May 2, 2021
WEB OF SCIENCETM
checked on Dec 6, 2021
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.