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Title: Infrapatellar Fat Pad Gene Expression and Protein Production in Patients with and without Osteoarthritis
Authors: Belluzzi, Elisa 
Macchi, Veronica 
Fontanella, Chiara Giulia 
Carniel, Emanuele Luigi
Olivotto, Eleonora
Filardo, Giuseppe
Sarasin, Gloria
Porzionato, Andrea
Granzotto, Marnie
Pozzuoli, Assunta
Berizzi, Antonio 
Scioni, Manuela
De Caro, Raffaele
Ruggieri, Pietro 
Vettor, Roberto
Ramonda, Roberta
Rossato, Marco 
Favero, Marta 
Keywords: adipocyte;anterior cruciate ligament;inflammation;infrapatellar fat pad;osteoarthritis
Issue Date: 2020
Publisher: MDPI
Journal: International journal of molecular sciences 
Osteoarthritis (OA) is one of the most common joint disorders. Evidence suggests that the infrapatellar fat pad (IFP) is directly involved in OA pathology. However, a comparison between OA versus non-OA IFP is still missing. Thus, the aim of this study was to compare IFP molecular, adipocytes and extracellular matrix characteristics of patients affected by OA, and patients undergoing anterior cruciate ligament (ACL) reconstruction. We hypothesized that not only inflammation but also changes in adipocytes and extracellular matrix (ECM) composition might be involved in OA pathogenesis. Fifty-three patients were enrolled. IFP biopsies were obtained, evaluating: (a) lymphocytic infiltration and vascularization; (b) adipocytes area and number; (c) adipo-cytokines and extracellular matrix gene expression levels; (d) IL-6 and VEGF protein production; (e) collagen fibers distribution. OA IFP was more inflamed and vascularized compared to ACL IFP. OA IFP adipocytes were larger and numerically lower (1.3-fold) than ACL IFP adipocytes. An increase of gene expression of typical white adipose tissue genes was observed in OA compared to ACL IFP. Collagen-types distribution was different in the OA IFP group compared to controls, possibly explaining the change of the biomechanical characteristics found in OA IFP. Statistical linear models revealed that the adipocyte area correlated with BMI in the OA group. In conclusion, inflammation and fibrotic changes of OA IFP could represent novel therapeutic targets to counteract OA.
ISSN: 16616596
DOI: 10.3390/ijms21176016
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